Two senior executives - including Gillon McLachlan's right hand man - have left the AFL after the revelation of inappropriate relationships with younger women in the industry.
The sport's governing body was thrown into disrepute on Friday after the shock resignations of football manager Simon Lethlean and commercial boss Richard Simkiss.
Lethlean was seen as second only to McLachlan in the AFL hierarchy.
Both engaged in "inappropriate relationships with younger women that work in the AFL industry", though McLachlan chose not to outline the indiscretions further.
"As CEO I am dealing with the workplace issues ... the people involved are allowed to deal with the personal and family matters out of the public sphere," he said.
"Senior leaders in our game sign up for the public scrutiny. Families and others do not. I ask their privacy be respected."
The bombshell resignations come a week after the sport's diversity manager Ali Fahour left the AFL for an assault at a suburban football match.
McLachlan said he became aware of the "pertinent facts" around Lethlean and Simkiss' actions this week, and they resigned late on Thursday night.
McLachlan declined to answer whether he had asked for their resignations.
"These two men own the decision to resign today. But it is a decision that I think is the right one," he said.
The bombshell announcements call into question the culture at the sport's head office.
But McLachlan, who has committed to improving gender equality as a 'Male Champion of Change' said his actions showed the sport's seriousness on women's issues.
"I'm proud of the change we're making. We have made dramatic change - whether it is with the amount of women on my management team (or) the amount of women in the organisation, talented women," he said.
"We feel that we've addressed the issues strongly today. And we'll continue to make change because it's a journey and we're not where we want to be yet."
Lethlean was appointed to his role in March this year as Mark Evans' replacement after serving in several high-ranking roles with the league.
Simkiss has worked with the AFL for more than a decade.
The pair have been replaced by two internal candidates in an acting capacity.
Darren Birch will fill the commercial role, while general counsel Andrew Dillon will add the wide-ranging football responsibilities to his workload.
McLachlan said he would look to bring in someone from outside the AFL head office to fill Lethlean's role.